A few more things.
Before you divide your 1000 feet of tubing into four equal lengths, take out a length that would route from the manifold area down into the floor, out to almost the coldest spot, like a few feet in from a door on a north wall. Press a plug (like a 1/2" bolt) into the end to plug it, and tie it into the re-rod grid just like you will do with your heat tubes. Later, when wiring, you will push a slab temp sensor on its wires, down this tube with a snake, to get a temperature reading for your thermostat. This way, your system seeks to keep the slab at the same temp all the time, instead of responding to every cool breeze that happens when the door opens momentarily. Fewer cycles, longer operation each cycle, so more efficient, and more even temps in room.
Attempt to get the tubing about centered in the slab vertically. It would be most correct to put your re-rod grid up an inch and a half, then tubing tied to the top of that, if pouring about 5 inches thick.
I used a deal called a Easy-route. It controls and routes the tubes into the slab and makes it more resistant to damage on floor pouring day. Google that.
Once it is all ready for concrete, hook it all up to a manifold with a pressure gauge on it and a shraeder valve so you can pressurize it with air. Put 15-20 lbs in it and watch it for a day. It should not go down more than a pound due to relaxation of the tube shape. Leave pressure in it while you pour concrete, keep glancing at it. If pressure suddenly drops, the crete workers have damaged the tube. STOP them, have one put the air onto the shraeder valve, and it will bubble out wherever the damage is. Cut the tube on the damage point. Use your splice kit (a double-ended compression fitting in brass), to repair. Have two splice kits in your pocket, along with wrenches. If you have them, you wont need them.
Use a concrete pump if possible. WAY easier on the tubing to have guys walking around on it, compared to having them dump wheelbarrows on it (that stresses me out just thinking about it).