What did it die from?
It's the surfactant in the glyphosphate mixtures that kills the fish, which is why there are some formulations for aquatic use.
What did it die from?
Just remember, the genes added to crops to give them resistance to Roundup are mobile and over time, will cross, and have crossed, to various weeds.
If you spray and nothing happens, either you forgot to add the chemicals, or you've got resistant weeds.