I owned semi trucks for 28 years. I now own two diesel mowers and a diesel tractor. With the exception of adding #1 for winter blend in the snow blower "mower" I have never to the best of my knowledge blended #1 in anything else. I live in MN and while I did have a gell up and added 911 to a tank (due to a faulty fuel heater) I have never used an additive. BTW the 911 was a complete waste of money.
There are thousands of of big trucks on the road and they buy millions of truck stop diesel, none of which has an additive. As for Bio, the higher the bio percentage the lower the power and mileage, and the lower the gell temp. That said it’s a good marketing ploy for the farmers!
One of my customers spent a summer buying and running 99.9 bio in their trucks. We would load 6993 of bean oil and then pump 7 gallons of #2 out of a gas boy barrel pump into the trailer. While it was at the time $.30 a gallon cheaper than straight 2 the mileage and power drop was significant. The shop foreman put a quart of it in the fridge and said it started to cloud at 37 degrees.