I've run both. The 3200 and L3301, and the L3800 and the L3901 so I can compare them.
The 01's have more power and it's obvious once you load the engine. The 01's governor is electronic. When bush hogging, I was watching the PTO speed...no load cutter turned off, engine was at 2560 RPM. Turn the bush hog on and RPM drops initially to around 2500, then recovers and holds 2550-2560. Loaded, it stays right around 2560 RPM until the load overcomes the available engine torque, which honestly is pretty tough especially on the 3901. The engine is quieter than the 00 series. The engine itself is simpler...no governor inside the engine and one less camshaft. Only one now instead of two. The L01's are not just 3200 and 3800 engines with electronic injection-they are almost altogether different. Common rail electronic injection, injector placement is way different, cylinder head different, pistons different, etc. It is superior to the L00's in that sense. The tractor itself has a more curved hood, and you get a little better visibility if you're using the loader. Speaking of loader-the LA525 does have a slightly higher lift capacity than the LA524 that came on the L3200/3800. Splitting hairs there.
The complexity is something that owners are all concerned about and as a technician that deals with them, I am concerned on behalf of the owners. Thus far, there have been some issues with crank sensors and errors between the sensor and ECU, but we "think" Kubota has those issues ironed out. There will most likely be a campaign to fix the early ones.
The L01 3 point hitch is very slightly different in reference to the position control valve, but it's still almost the same as the L00's were. Just minor difference and smooths the lift a little. The jerky lifts on the 3800's were usually due to (1) it was a "cheap" tractor (lacked the shock control that the Grand L series had) and (2) some of them had a misadjusted position control valve. Or (3) the combination of the 2.
What I didn't like about the L01 series is the cheap plastic seat. It flexes and if you turn around to the right, the seat flexes just enough to lift the OPC switch and kill the engine. Easy fix but aggravating to have to deal with it. DPF is here to stay from here on out. Lot of people went out and bought the last of the 3200/3800's and that's all fine, but what happens when the 3200 they just bought is no longer supported by Kubota due to age and/or government intervention? Which we are already seeing (government intervention...) Might as well get used to the DPF. Or keep buying used tractors.
THe L2501 is also out...and gets rave reviews. It's an L3200 that turns less RPM. Does not have CRS and DPF, it is an L3200 with minor changes in the transmission to accomodate the reduced engine speed. Been popular here. Is it possible to make an L2501 in to an L3200? Absolutely-but there are a lot of parts to change and a lot of work to do it. Governor arm/fork, pump, springs, stoppers, then gearing inside of the transmsission, etc. More work than it's worth.
Absolutely nothing wrong at all with the L01's now that the issues with the early ones have been addressed.
Using the DPF is really nothing to it. If you're not wanting to regen, hit the button (DPF inhibit). If you've got the motor at 2200+ RPM and under a load, say mowing or bush hogging, it regen's on it's own and you never know it. Other than the light in the dash comes on to let you know it's doing what it's supposed to. That's it. Nothing to it. You get more HP, more torque, better engine response to load changes, uses less fuel on average (does use more during regen). Downside-complexity. Time will tell how well the systems work but thus far, nary a problem. DPF will need to be cleaned at some point and as of right now it's not cheap-but as more and more tractors get them and they get pulled/cleaned more, the prices will come down. Maintenance of the engine is identical to the L3200 as far as oil changes, but you'll have to be sure to use the correct oil (they call for 5W40 CJ-4 rating). Use of other oil may cause faster DPF clogging and perhaps even more frequent regen and extreme cases, a DPF replacement. Trucks have had these systems for a few years and they're pretty well maintainance free for most people. Even though maintenance is about the same, you cant just neglect them like you can a 3200 and expect it to be as trouble free. It don't work that way. Exactly like comparing the old 7.3 Power-less-stroke engine to the 6.0. The 6.0 is a better engine by far in a lot of ways, but people with 6.0's found out that you just can't change oil every 5000 miles and run the pee out of it and expect it to last as long as the old T444E's. At least that's been our experience with them-and the same so far with the L01 series Kubota's.
Kubota didn't want to do the DPF, but they had to. Same story for all of the manufacturers. The DPF system has been in use and tested for a few years, since roughly 2008, on prototypes. Everyone knew it was coming. Everyone was able to buy credits to allow them to run Tier 3 and Interim Tier 4 engines but once those credits ran out they had no choice. So really it's not a new engine or new systems.